Journal «Angiology and Vascular Surgery» • 

2015 • VOLUME 21 • №1

Oxidative carbonylation of vascular wall proteins in dynamics of experimental venous thrombosis

Fomina N.V.1, Fomina M.A.1, Kalinin R.E.2, Suchkov I.A.2

1) Chair of Biological Chemistry with a course of Clinical Laboratory Diagnosis, Faculty of Additional Professional Education, Ryazan State Medical University named after Academician I.P. Pavlov under the RF Public Health Ministry,
2) Chair of Angiology, Vascular, Operative Surgery and Topographical Anatomy, Ryazan State Medical University named after Academician I.P. Pavlov under the RF Public Health Ministry, Ryazan, Russia

The objects of the study were a total of 24 conventional sexually mature Wistar rats weighing 200-400 g. Thrombosis was modelled by means of ligation of the common iliac vein. Animals were withdrawn from the study on days 1, 3 and 5 after intervention. The materials for the study in each animal were homogenates of the vein portion below the site of ligation (thrombosed vein) and the portion of the symmetrical vessel (intact vein). Taken as controls were portions of the common iliac vein of intact animals matched by age, body weight, and keeping conditions.

The level of spontaneous and induced in the Fenton reaction oxidative carbonylation of proteins was determined by means of carbonyl derivatives according to the R.L. Levine technique modified by E.E. Dubinina with optical registration of the formed dinitrophenylhydrazines at 356, 370, 430 and 530 nm. The reserve-and-adaptation potential was assessed by means of counting the ratio of the amount of carbonyl derivatives of proteins in spontaneous and induced oxidation.

The obtained findings showed that experimental thrombosis is accompanied and followed by an increase in the content of carbonyl derivatives of proteins in the wall of thrombosed veins and, to a lesser degree, in that of intact veins. The maximal elevation of the parameters was registered during the first 24 hours of the development of pathology, demonstrating not only early but late markers of oxidative modification of proteins. Thrombosed veins on day 3 were found to have a decrease in the content of carbonylated proteins to the level of the control values, which was associated with a maximal value of the reserve-adaptation potential. However, day five was marked by a secondary increase in carbonylation accompanied by certain exhaustion of the reserve-adaptive potential. In intact veins, a decrease of the spontaneous oxidative modification level on day 3 was accompanied by a splash of induced carbonylation followed by stabilization of the parameters at the levels slightly exceeding the control values by day 5.

KEY WORDS: protein oxidative carbonylation, experimental venous thrombosis, vascular wall.

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